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primary antibodies against cd86  (Bioss)


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    Bioss primary antibodies against cd86
    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type <t>(CD86)</t> and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.
    Primary Antibodies Against Cd86, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+cd86/CD86+Polyclonal+Antibody/pmc12883363-131-0-6
    Average 95 stars, based on 129 article reviews
    primary antibodies against cd86 - by Bioz Stars, 2026-10
    95/100 stars

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    1) Product Images from "Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway"

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1666920

    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type (CD86) and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.
    Figure Legend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type (CD86) and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.

    Techniques Used: In Vivo, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vitro . (A–D) Elisa was used to examine the expression levels of NLRP3, IL-6, TNF-α and IL-10 expression level. (E–G) Immunofluorescence staining of Iba-1, CD86, CD206. (H–J) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ****P < 0.0001.
    Figure Legend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vitro . (A–D) Elisa was used to examine the expression levels of NLRP3, IL-6, TNF-α and IL-10 expression level. (E–G) Immunofluorescence staining of Iba-1, CD86, CD206. (H–J) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ****P < 0.0001.

    Techniques Used: In Vitro, Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining

    The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Techniques Used: In Vitro, Immunofluorescence, Staining

    Related Articles

    Incubation:

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway
    Article Snippet: After cooling to room temperature, nonspecific binding was blocked by applying 5% normal goat serum (diluted 1:19 in PBS; SL038; Solarbio) for 30 min, followed by gentle washing. .. Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C. .. After washing, the sections were incubated with appropriate secondary antibodies: CyTM3-conjugated goat anti-rabbit IgG ( GB113497 ; Jackson) and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG (GB22303; Servicebio) for 50 min at room temperature.

    Article Title: Inhibition of HMGN2 SUMOylation Ameliorates Atherosclerosis by Activating PAX5 Expression to Induce Macrophage M2 Polarization
    Article Snippet: Background: As a core pathological process of cardiovascular disease, atherosclerosis (AS) progression is closely linked to macrophage polarization, yet the mechanisms by which post-translational modifications regulate inflammatory responses in AS remain unclear.. Methods: Using oxidized low-density lipoprotein (ox-LDL)-induced RAW264.7 foam macrophages and high-fat diet-fed (apolipoprotein E knockout) ApoE /− mice, we assessed HMGN2 SUMOylation’s role in macrophage M2 polarization.. Cell proliferation/migration were analyzed via EdU/Transwell assays; macrophage polarization phenotypes were examined by immunofluorescence.



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    primary antibodies against cd86  (Bioss)
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    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type <t>(CD86)</t> and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.
    Primary Antibodies Against Cd86, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Chemical structure of ATG. (B) Target prediction of ATG. (C) Molecular targets associated with MAFLD. (D) Intersection of ATG-specific and MAFLD-related targets. (E) The PPI network of intersection targets between ATG and MAFLD-related targets. (F) GO enrichment analysis of the intersected targets. (G–H) Enrichment of KEGG pathway (G) and Reactome pathway (H) of ATG’s targets. (I–J) Immunofluorescence analysis of the colocalization of NLRP3 and <t>CD86</t> in mice liver from four groups (scale bar: 20 µm), staining intensity was quantified, and colocation analysis was performed. (K) NLRP3 with ATG bound to the cavity. (L) Western blot of CETSA experiment to further confirm the interaction between NLRP3 and ATG in RAW264.7 cells, the temperature ranges from 49°C to 61°C, with relative band intensity normalized to 49°C. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ATG, arctigenin; MAFLD, metabolic dysfunction-associated fatty liver disease; PPI, protein-protein interaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; NLRP3, NLR family pyrin domain containing 3; SEM, standard error of the mean.
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    primary antibodies against cd86  (Proteintech)
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    (A) Chemical structure of ATG. (B) Target prediction of ATG. (C) Molecular targets associated with MAFLD. (D) Intersection of ATG-specific and MAFLD-related targets. (E) The PPI network of intersection targets between ATG and MAFLD-related targets. (F) GO enrichment analysis of the intersected targets. (G–H) Enrichment of KEGG pathway (G) and Reactome pathway (H) of ATG’s targets. (I–J) Immunofluorescence analysis of the colocalization of NLRP3 and <t>CD86</t> in mice liver from four groups (scale bar: 20 µm), staining intensity was quantified, and colocation analysis was performed. (K) NLRP3 with ATG bound to the cavity. (L) Western blot of CETSA experiment to further confirm the interaction between NLRP3 and ATG in RAW264.7 cells, the temperature ranges from 49°C to 61°C, with relative band intensity normalized to 49°C. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ATG, arctigenin; MAFLD, metabolic dysfunction-associated fatty liver disease; PPI, protein-protein interaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; NLRP3, NLR family pyrin domain containing 3; SEM, standard error of the mean.
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    (A) Chemical structure of ATG. (B) Target prediction of ATG. (C) Molecular targets associated with MAFLD. (D) Intersection of ATG-specific and MAFLD-related targets. (E) The PPI network of intersection targets between ATG and MAFLD-related targets. (F) GO enrichment analysis of the intersected targets. (G–H) Enrichment of KEGG pathway (G) and Reactome pathway (H) of ATG’s targets. (I–J) Immunofluorescence analysis of the colocalization of NLRP3 and <t>CD86</t> in mice liver from four groups (scale bar: 20 µm), staining intensity was quantified, and colocation analysis was performed. (K) NLRP3 with ATG bound to the cavity. (L) Western blot of CETSA experiment to further confirm the interaction between NLRP3 and ATG in RAW264.7 cells, the temperature ranges from 49°C to 61°C, with relative band intensity normalized to 49°C. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ATG, arctigenin; MAFLD, metabolic dysfunction-associated fatty liver disease; PPI, protein-protein interaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; NLRP3, NLR family pyrin domain containing 3; SEM, standard error of the mean.
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    (A) Chemical structure of ATG. (B) Target prediction of ATG. (C) Molecular targets associated with MAFLD. (D) Intersection of ATG-specific and MAFLD-related targets. (E) The PPI network of intersection targets between ATG and MAFLD-related targets. (F) GO enrichment analysis of the intersected targets. (G–H) Enrichment of KEGG pathway (G) and Reactome pathway (H) of ATG’s targets. (I–J) Immunofluorescence analysis of the colocalization of NLRP3 and <t>CD86</t> in mice liver from four groups (scale bar: 20 µm), staining intensity was quantified, and colocation analysis was performed. (K) NLRP3 with ATG bound to the cavity. (L) Western blot of CETSA experiment to further confirm the interaction between NLRP3 and ATG in RAW264.7 cells, the temperature ranges from 49°C to 61°C, with relative band intensity normalized to 49°C. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ATG, arctigenin; MAFLD, metabolic dysfunction-associated fatty liver disease; PPI, protein-protein interaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; NLRP3, NLR family pyrin domain containing 3; SEM, standard error of the mean.
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    Image Search Results


    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type (CD86) and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

    doi: 10.3389/fimmu.2025.1666920

    Figure Lengend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type (CD86) and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.

    Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

    Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vitro . (A–D) Elisa was used to examine the expression levels of NLRP3, IL-6, TNF-α and IL-10 expression level. (E–G) Immunofluorescence staining of Iba-1, CD86, CD206. (H–J) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ****P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

    doi: 10.3389/fimmu.2025.1666920

    Figure Lengend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vitro . (A–D) Elisa was used to examine the expression levels of NLRP3, IL-6, TNF-α and IL-10 expression level. (E–G) Immunofluorescence staining of Iba-1, CD86, CD206. (H–J) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ****P < 0.0001.

    Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

    Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining

    The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

    doi: 10.3389/fimmu.2025.1666920

    Figure Lengend Snippet: The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

    Techniques: In Vitro, Immunofluorescence, Staining

    (A) Chemical structure of ATG. (B) Target prediction of ATG. (C) Molecular targets associated with MAFLD. (D) Intersection of ATG-specific and MAFLD-related targets. (E) The PPI network of intersection targets between ATG and MAFLD-related targets. (F) GO enrichment analysis of the intersected targets. (G–H) Enrichment of KEGG pathway (G) and Reactome pathway (H) of ATG’s targets. (I–J) Immunofluorescence analysis of the colocalization of NLRP3 and CD86 in mice liver from four groups (scale bar: 20 µm), staining intensity was quantified, and colocation analysis was performed. (K) NLRP3 with ATG bound to the cavity. (L) Western blot of CETSA experiment to further confirm the interaction between NLRP3 and ATG in RAW264.7 cells, the temperature ranges from 49°C to 61°C, with relative band intensity normalized to 49°C. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ATG, arctigenin; MAFLD, metabolic dysfunction-associated fatty liver disease; PPI, protein-protein interaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; NLRP3, NLR family pyrin domain containing 3; SEM, standard error of the mean.

    Journal: Journal of Clinical and Translational Hepatology

    Article Title: Arctigenin Prevents Metabolic Dysfunction-associated Steatohepatitis by Inhibiting NLRP3/GSDMD-N Axis in Macrophages

    doi: 10.14218/JCTH.2025.00141

    Figure Lengend Snippet: (A) Chemical structure of ATG. (B) Target prediction of ATG. (C) Molecular targets associated with MAFLD. (D) Intersection of ATG-specific and MAFLD-related targets. (E) The PPI network of intersection targets between ATG and MAFLD-related targets. (F) GO enrichment analysis of the intersected targets. (G–H) Enrichment of KEGG pathway (G) and Reactome pathway (H) of ATG’s targets. (I–J) Immunofluorescence analysis of the colocalization of NLRP3 and CD86 in mice liver from four groups (scale bar: 20 µm), staining intensity was quantified, and colocation analysis was performed. (K) NLRP3 with ATG bound to the cavity. (L) Western blot of CETSA experiment to further confirm the interaction between NLRP3 and ATG in RAW264.7 cells, the temperature ranges from 49°C to 61°C, with relative band intensity normalized to 49°C. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ATG, arctigenin; MAFLD, metabolic dysfunction-associated fatty liver disease; PPI, protein-protein interaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; NLRP3, NLR family pyrin domain containing 3; SEM, standard error of the mean.

    Article Snippet: Primary antibodies against CD86 (19589, Cell Signaling Technology, CST®; 1:400 in 3% BSA) and α-SMA (ab124964, Abcam®; 1:2,000 in 3% BSA) were incubated overnight.

    Techniques: Immunofluorescence, Staining, Western Blot